Drug discovery screening based on epigenetic control of COPD – Benserazide inhibits the prothymosin α-H1 histone interaction

Prothymosin alpha modulates the interaction of histone H1 with chromatin

Prothymosin alpha (ProTalpha) is an abundant acidic nuclear protein that may be involved in cell proliferation. In our search for its cellular partners, we have recently found that ProTalpha binds to linker histone H1. We now provide further evidence for the physiological relevance of this interaction by immunoisolation of a histone H1-ProTalpha complex from NIH 3T3 cell extracts. A detailed an...

Epigenetic control of the immune system: histone demethylation as a target for drug discovery.

In recent years significant progress has been made in our understanding of epigenetic control of a wide range of cellular processes. This has come about both through the concerted effort of the research community and through the development of technologies essential to the area. The importance of the epigenetic control of the immune system is becoming increasingly clear, and therefore epigeneti...

The Unexpected Effect of Sodium Arsenate on the Interaction between Histone H1 and Sodium N-Dodecyl Sulphate

A Study was made on the interaction between histon H1 and sodium n-dodecyl sulphate (SDS) in the presence of sodium arsenate inside a phosphate buffer of pH 6.4, using spectroscopy and equilibrium dialysis at 27 °C. The binding data has been used to obtain the gibbs free energy in terms of a theoretical model based on the Wyman binding potential. The binding data hs been analysed...

Epigenetic targets and drug discovery: part 1: histone methylation.

Dynamic chromatin structure is modulated by post-translational modifications on histones, such as acetylation, phosphorylation and methylation. Research on histone methylation has become the most flourishing area of epigenetics in the past fourteen years, and a large amount of data has been accumulated regarding its biology and disease implications. Correspondingly, a lot of efforts have been m...

***Removed from the Issue*** Application of Cell-Based Assay Systems for the Early Screening of Human Drug Hepatotoxicity in the Discovery Phase of Drug Development